sdhA

sdhA
168

succinate dehydrogenase (flavoprotein subunit)

Locus
BSU_28440
Molecular weight
65.17 kDa
Isoelectric point
5.71
Protein length
586 aaSequenceBlast
Gene length
1761 bpSequenceBlast
Function
TCA cycle
Product
succinate dehydrogenase (flavoprotein subunit)
Essential
no
Synonyms
sdhA, citF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1053 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,906,335  2,908,095
Phenotypes of a mutant
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C PubMed
The protein
Catalyzed reaction/ biological activity
menaquinone + succinate --> menaquinol + fumarate PubMed
menaquinone + malate --> menaquinol + enol-oxaloacetate (minor side reaction) PubMed
Protein family
FAD-dependent oxidoreductase 2 family (with NadB, according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
1NEK (PDB) (E. coli) PubMed
P08065 (AlphaFold)
Modification
phosphorylated PubMed, PubMed
Effectors of protein activity
Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
Inhibited by enroll-oxaloacetate PubMed
Activated by Cytochrome b558 PubMed
Localization
attached to the membrane PubMed
Expression and Regulation
Operons
Genes
sdhC-sdhA-sdhB
Description
PubMed
Regulation
constitutive
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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sdhC→sdhB

2025-04-07 15:26:31

ghost

86

6e4216337509de45d76033b51f5046e391052666

7F08B53F36BD96778ADC5652AD8BF65AF07550E2

Other regulations
FsrA: translation inhibition, PubMed
Biological materials
Mutant
GP743 ∆(sdhC-sdhA), cat, available in Jörg Stülke's lab
GP792 ∆(sdhC-sdhA-sdhB)::phleo, available in Jörg Stülke's lab
GP2342 ∆(sdhC-sdhA-sdhB)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
GP2343 ∆(sdhC-sdhA-sdhB)::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
BKE28440 ∆(sdhA)::erm  trpC2, available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC,  downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA
BKK28440 ∆(sdhA::kan)  trpC2, available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC,  downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E

Page visits: 4933

Time of last update: 2025-04-08 18:10:07

Author of last update: Jstuelk