mecA

mecA
168

buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK and ComS to the ClpC-ClpP degradation machine (in log phase), inhibits the transcriptional activity of Spo0A-P by direct interaction

Locus
BSU_11520
Molecular weight
25.62 kDa
Isoelectric point
4.21
Protein length
218 aaSequenceBlast
Gene length
657 bpSequenceBlast
Function
control of ComK degradation, regulation of competence
Product
adaptor protein
Essential
no
Synonyms
mecA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4862 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,229,068  1,229,724
Phenotypes of a mutant
defective in swarming motility (reduced rate of colony expansion) PubMed
The protein
Catalyzed reaction/ biological activity
recruits protein for degradation by ClpC-ClpP PubMed
inhibits transcription activation of Spo0A-P (in complex with ClpC) PubMed
Protein family
mecA family (with YpbH, according to UniProt)
Domains
N-terminal domain (1-120): recruitment of protein substrates PubMed
C-terminal domain (121-218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed
Structure
3J3T (PDB) (MecA6-ClpC6 complex) PubMed
3JTP (PDB)(C-terminal domain) PubMed
P37958 (AlphaFold)
Paralogous protein(s)
YpbH
Expression and Regulation
Operons
Genes
mecA
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mecA

2025-04-05 06:04:55

ghost

76

a7eff32f2142ccd5507e9841a00a021df33d8346

9393514154CA17D711EA90973907CF0CBFF6C1FF

Biological materials
Mutant
GP813 (spc), GP814 (aphA3) both available in Jörg Stülke's lab
BKE11520 (mecA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC,  downstream forward: _UP4_TAGCAAACCGATTTCCTTCC
BKK11520 (mecA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC,  downstream forward: _UP4_TAGCAAACCGATTTCCTTCC
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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331993A875907C10C77105FD8DDD86D4412CE405

Page visits: 6513

Time of last update: 2025-04-05 12:49:30

Author of last update: Jstuelk