mtlF

mtlF
168

mannitol-specific permease of the phosphotransferase system, EIIA of the PTS

Locus
BSU_03982
Molecular weight
16.00 kDa
Isoelectric point
4.496
Protein length
143 aaSequenceBlast
Gene length
432 bpSequenceBlast
Function
mannitol uptake and phosphorylation
Product
mannitol-specific PTS, EIIA
Essential
no
Synonyms
mtlF
Outlinks
BsubCyc UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4668 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
451,185 → 451,616
The protein
Protein family
PTS permease, fructose/ mannitol family PubMed
Domains
PTS EIIA domain type-2 (aa 2-142) (according to UniProt)
Structure
1A3A (PDB) (from E. coli, 41% identity) PubMed
C0H3V2 (AlphaFold)
Expression and Regulation
Operons
Genes
mtlA-mtlF-mtlD
Description
PubMed
Regulation
induced by mannitol (MtlR) PubMed
Regulatory mechanism
MtlR: activation, PubMed, in mtlR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for mtlD PubMed
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mtlA→mtlD

2025-04-02 10:20:37

Jstuelk

68

5bae19b2d80b020443468ffbe472a91dbcc86cee

F44226BCF3EE1A7B00EEDFF583F5DD4C12739D38

Biological materials
Mutant
BKE03982 (ΔmtlF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACAATCACTCTCTTTC,  downstream forward: _UP4_GCCATTTTCAACGAGGTGAA
BKK03982 (ΔmtlF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACAATCACTCTCTTTC,  downstream forward: _UP4_GCCATTTTCAACGAGGTGAA
References
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Time of last update: 2025-04-07 02:27:53

Author of last update: Jstuelk