YabA

• Description: inhibitor of DnaA oligomerization, tethers DnaA to the polymerase-clamp protein DnaN
  
  

Gene name	yabA
Synonyms
Essential	no
Product	negative regulator of replication initiation
Function	positioning of DnaA
Gene expression levels in SubtiExpress: yabA
Interactions involving this protein in SubtInteract: YabA
MW, pI	13 kDa, 6.065
Gene length, protein length	357 bp, 119 aa
Immediate neighbours	yaaT, yabB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication

The gene

Basic information

• Locus tag: BSU00330

Phenotypes of a mutant

strongly reduced growth rate in minimal medium PubMed

Database entries

• BsubCyc: BSU00330

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • negative control of replication initiation PubMed
  • associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed
  • YabA inhibits co-operative DNA binding by DnaA PubMed
  • inhibits oligomerization and helix formation of DnaA PubMed

• Protein family: yabA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: leucine zipper

• Modification:

• Cofactor(s): Zn cluster

• Effectors of protein activity:
  • DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed

• Interactions: DnaA-YabA-DnaN PubMed

• Localization:

Database entries

• BsubCyc: BSU00330

• Structure:

• UniProt: P37542

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yaaQ-yaaR-holB-yaaT-yabA-yabB-yazA-yabC PubMed

• Expression browser: yabA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 199 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France Homepage
Alan Grossman, Cambridge, MA, USA Homepage

References

Reviews

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)