PhoR

• Description: two-component sensor kinase, regulation of phosphate metabolism
  
  

Gene name	phoR
Synonyms
Essential	no
Product	two-component sensor kinase
Function	regulation of phosphate metabolism
Gene expression levels in SubtiExpress: phoR
Interactions involving this protein in SubtInteract: PhoR
MW, pI	64 kDa, 5.957
Gene length, protein length	1737 bp, 579 aa
Immediate neighbours	polA, phoP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, SigB regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU29100

Database entries

• BsubCyc: BSU29100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of PhoP

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PhoP-PhoR

• Localization:

Database entries

• BsubCyc: BSU29100

• Structure: 3CWF (extracellular PAS domain) PubMed

• UniProt: P23545

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: phoP-phoR PubMed

• Expression browser: phoR PubMed

• Sigma factor: SigA PubMed, SigB PubMed, SigE PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • expressed in post-exponential phase (ScoC) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • PhoP: transcription activation PubMed
  • ScoC: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 453 PubMed

Biological materials

• Mutant:
  • 1A966 ( phoR::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage