CysH

• Description: phosphoadenosine phosphosulfate sulfotransferase
  
  

Gene name	cysH
Synonyms
Essential	no
Product	phosphoadenosine phosphosulfate sulfotransferase
Function	sulfate reduction
Gene expression levels in SubtiExpress: cysH
Metabolic function and regulation of this protein in SubtiPathways: cysH
MW, pI	26 kDa, 5.462
Gene length, protein length	699 bp, 233 aa
Immediate neighbours	pyrE, cysP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

• Locus tag: BSU15570

Database entries

• BsubCyc: BSU15570

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)

• Protein family: CysH subfamily (according to Swiss-Prot)

• Paralogous protein(s): YitB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15570

• Structure: 2GOY (from Pseudomonas aeruginosa, 35% identity) PubMed

• UniProt: P94498

• KEGG entry: [3]

• E.C. number: 1.8.4.8

Expression and regulation

• Operon: cysH-cysP-sat-cysC-ylnD-ylnE-ylnF PubMed

• Expression browser: cysH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed in the presence of cysteine (CymR)
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism:
  • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • CymR: transcription repression

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 520 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France