Rph

• Description: RNase PH, 3'-5' exoribonuclease
  
  

Gene name	rph
Synonyms
Essential	no
Product	RNase PH (EC 2.7.7.56)
Function	3'-5' exoribonuclease
Gene expression levels in SubtiExpress: rph
MW, pI	26 kDa, 4.888
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	ysnA, gerM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation

The gene

Basic information

• Locus tag: BSU28370

Database entries

• BsubCyc: BSU28370

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
  • responsible for endonucleolytic cleavage of scRNA (scr) PubMed

• Protein family: RNase PH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28370

• Structure: 1OYS

• UniProt: P28619

• KEGG entry: [2]

• E.C. number: 2.7.7.56

Expression and regulation

• Operon:

• Expression browser: rph PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 274 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1204 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage