FadB

• Description: 3-hydroxyacyl-CoA dehydratase
  
  

Gene name	fadB
Synonyms	ysiB
Essential	no
Product	3-hydroxyacyl-CoA dehydratase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadB
Metabolic function and regulation of this protein in SubtiPathways: fadB
MW, pI	27 kDa, 4.951
Gene length, protein length	774 bp, 258 aa
Immediate neighbours	etfB, fadR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

• Locus tag: BSU28540

Database entries

• BsubCyc: BSU28540

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H₂O (according to Swiss-Prot)

• Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-230 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28540

• Structure: 3PEA (from B. anthracis, 49% identity, 66% similarity)

• UniProt: P94549

• KEGG entry: [3]

• E.C. number: 4.2.1.17

Expression and regulation

• Operon:
  • lcfA-fadR-fadB-etfB-etfA PubMed
  • fadR-fadB-etfB-etfA PubMed

• Expression browser: fadB PubMed

• Sigma factor:
  • lcfA: SigA PubMed
  • fadR: SigA PubMed

• Regulation:
  • induced by long-chain fatty acids (FadR) PubMed
  • subject to carbon catabolite repression (3.8-fold) (CcpA-HPr(Ser-P) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 165 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 665 PubMed

Biological materials

• Mutant:
  • 1A854 ( fadB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed