TufA

• Description: elongation factor Tu
  
  

Gene name	tufA
Synonyms
Essential	yes PubMed
Product	elongation factor Tu
Function	translation
Gene expression levels in SubtiExpress: tufA
Interactions involving this protein in SubtInteract: TufA
MW, pI	43 kDa, 4.723
Gene length, protein length	1188 bp, 396 aa
Immediate neighbours	fusA, ybaC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, membrane proteins, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01130

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01130

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: EF-Tu/EF-1A subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Thr-384 by PrkC, dephosphorylated by PrpC PubMed, PubMed
  • Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species PubMed
  • phosphorylated on several Arg residues PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • MreB-TufA PubMed

• Localization:
  • EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB PubMed
  • membrane PubMed

Database entries

• BsubCyc: BSU01130

• Structure:

• UniProt: P33166

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: tufA PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 59691 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 237848 PubMed

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP839, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP847, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: