Lgt

• Description: prolipoprotein diacylglyceryl transferase
  
  

Gene name	lgt
Synonyms	gerF, yvoC
Essential	no
Product	prolipoprotein diacylglyceryl transferase
Function	lipomodification of lipoproteins
Gene expression levels in SubtiExpress: lgt
Interactions involving this protein in SubtInteract: Lgt
Metabolic function and regulation of this protein in SubtiPathways: lgt
MW, pI	30 kDa, 8.96
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	yvoD, hprK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, germination, membrane proteins

The gene

Basic information

• Locus tag: BSU34990

Phenotypes of a mutant

• reduced ability of spores to germinate with alanine due ti reduced activity of the GerAA-GerAB-GerAC germinant receptor PubMed

Database entries

• BsubCyc: BSU34990

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lgt family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerAC-Lgt to fix GerAC to diacylglycerol in the membrane PubMed
  • GerD-Lgt to fix GerD to diacylglycerol in the membrane PubMed

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU34990

• Structure:

• UniProt: O34752

• KEGG entry: [2]

• E.C. number: 2.4.99.-

Expression and regulation

• Operon: hprK-lgt-yvoD-yvoE-yvoF PubMed

• Expression browser: lgt PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 139 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 391 PubMed

Biological materials

• Mutant: GP851 (deletion of lgt-yvoD-yvoE-yvoF, replaced by aphA3), available in the Stülke lab
  • 1G22 ( lgt::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

PubMed

Lgt in other organisms

PubMed