HemL

• Description: glutamate-1-semialdehyde aminotransferase
  
  

Gene name	hemL
Synonyms	hemK
Essential	no
Product	glutamate-1-semialdehyde aminotransferase
Function	heme biosynthesis
Gene expression levels in SubtiExpress: hemL
Metabolic function and regulation of this protein in SubtiPathways: HemL
MW, pI	46 kDa, 5.055
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	spoVID, hemB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU28120

Database entries

• BsubCyc: BSU28120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-4-amino-5-oxopentanoate = 5-aminolevulinate (according to Swiss-Prot)

• Protein family: HemL subfamily (according to Swiss-Prot)

• Paralogous protein(s): GsaB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28120

• Structure: 3BS8 PubMed

• UniProt: P30949

• KEGG entry: [3]

• E.C. number: 5.4.3.8

Expression and regulation

• Operon: hemA-hemX-hemC-hemD-hemB-hemL PubMed

• Expression browser: hemL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by hydrogen peroxide (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 762 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1928 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Honghua Ge, Xinhuai Lv, Jun Fan, Yongxiang Gao, Maikun Teng, Liwen Niu
Crystal structure of glutamate-1-semialdehyde aminotransferase from Bacillus subtilis with bound pyridoxamine-5'-phosphate.
Biochem Biophys Res Commun: 2010, 402(2);356-60
[PubMed:20946885] [WorldCat.org] [DOI] (I p)

A F Herbig, J D Helmann
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.
Mol Microbiol: 2001, 41(4);849-59
[PubMed:11532148] [WorldCat.org] [DOI] (P p)

Per Johansson, Lars Hederstedt
Organization of genes for tetrapyrrole biosynthesis in gram--positive bacteria.
Microbiology (Reading): 1999, 145 ( Pt 3);529-538
[PubMed:10217486] [WorldCat.org] [DOI] (P p)

M Hansson, L Rutberg, I Schröder, L Hederstedt
The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III.
J Bacteriol: 1991, 173(8);2590-9
[PubMed:1672867] [WorldCat.org] [DOI] (P p)