YlxS

• Description: similar to 30S ribosomal subunit maturation protein

Gene name	ylxS
Synonyms	ymxA
Essential	no
Product	unknown
Function	ribosome maturation
Gene expression levels in SubtiExpress: ylxS
MW, pI	17 kDa, 4.482
Gene length, protein length	468 bp, 156 aa
Immediate neighbours	polC, nusA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16590

Database entries

• BsubCyc: BSU16590

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0090 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU16590

• Structure:

• UniProt: P32726

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylxS-nusA-ylxR-ylxQ-infB-ylxP-rbfA PubMed

• Expression browser: ylxS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 254 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 442 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Stefan Nord, Göran O Bylund, J Mattias Lövgren, P Mikael Wikström
The RimP protein is important for maturation of the 30S ribosomal subunit.
J Mol Biol: 2009, 386(3);742-53
[PubMed:19150615] [WorldCat.org] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

K Shazand, J Tucker, M Grunberg-Manago, J C Rabinowitz, T Leighton
Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.
J Bacteriol: 1993, 175(10);2880-7
[PubMed:8491709] [WorldCat.org] [DOI] (P p)