HemAT

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  • Description: soluble chemotaxis receptor, heme-containing O(2) sensor protein

Gene name hemAT
Synonyms yhfV
Essential no
Product haem-based aerotactic transducer
Function movement towards oxygen
Gene expression levels in SubtiExpress: hemAT
Interactions involving this protein in SubtInteract: HemAT
MW, pI 48 kDa, 5.441
Gene length, protein length 1296 bp, 432 aa
Immediate neighbours yhfU, yhfW
Sequences Protein DNA DNA_with_flanks
Genetic context
HemAT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HemAT expression.png















Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU10380

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): heme
  • Effectors of protein activity:
  • Localization:
    • forms clusters at the cell poles PubMed
    • homogeneous cytoplsmic distribution in chain-forming cells during the logarithmic phase PubMed
    • polar foci in individual cells at later growth stages PubMed

Database entries

  • Structure: 1OR4 (in cyano-liganded form), 1OR6
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1095 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1803 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Markéta Martínková, Kenichi Kitanishi, Toru Shimizu
Heme-based globin-coupled oxygen sensors: linking oxygen binding to functional regulation of diguanylate cyclase, histidine kinase, and methyl-accepting chemotaxis.
J Biol Chem: 2013, 288(39);27702-11
[PubMed:23928310] [WorldCat.org] [DOI] (I p)

Original publications