YwaA

• Description: branched-chain amino acid aminotransferase
  
  

Gene name	ywaA
Synonyms	ipa-0r
Essential	no
Product	branched-chain amino acid aminotransferase
Function	biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ywaA
Metabolic function and regulation of this protein in SubtiPathways: ywaA
MW, pI	40 kDa, 4.952
Gene length, protein length	1089 bp, 363 aa
Immediate neighbours	dltE, licH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU38550

Database entries

• BsubCyc: BSU38550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s): YbgE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: S-cysteinylation after diamide stress (C104) PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU38550

• Structure: 3HT5 (from Mycobacterium tuberculosis, 42% identity, 58% similarity) PubMed

• UniProt: P39576

• KEGG entry: [3]

• E.C. number: 2.6.1.42

Expression and regulation

• Operon: ywaA PubMed

• Expression browser: ywaA PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1220 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1733 PubMed

Biological materials

• Mutant:
  • a ywaA::spc mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: