Zwf

• Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
  
  

Gene name	zwf
Synonyms	yqjJ
Essential	no
Product	glucose 6-phosphate dehydrogenase
Function	initiation of the pentose phosphate pathway
Gene expression levels in SubtiExpress: zwf
Metabolic function and regulation of this protein in SubtiPathways: zwf
MW, pI	55 kDa, 5.28
Gene length, protein length	1467 bp, 489 aa
Immediate neighbours	rnz, gndA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

The gene

Basic information

• Locus tag: BSU23850

Database entries

• BsubCyc: BSU23850

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)

• Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • Two Substrate Michaelis-Menten PubMed
  • specific activity: 0.375 µmol min^-1 (mg protein)^-1 PubMed

• Domains:

• Modification:

• Cofactors: Mg2+, Mn2+, Ca2+

• Effectors of protein activity:
  • NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23850

• Structure: 1DPG (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed

• UniProt: P54547

• KEGG entry: [3]

• E.C. number: 1.1.1.49

Additional information

The enzyme is a dimer PubMed

Expression and regulation

• Operon: zwf PubMed

• Expression browser: zwf PubMed

• Sigma factor:

• Regulation: constitutive PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2681 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3601 PubMed

Biological materials

• Mutant:
  • SM-NB3 (zwf-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1401 (spc, based on pGP1331), available in the Stülke lab

References

Michael Kohlstedt, Praveen K Sappa, Hanna Meyer, Sandra Maaß, Adrienne Zaprasis, Tamara Hoffmann, Judith Becker, Leif Steil, Michael Hecker, Jan Maarten van Dijl, Michael Lalk, Ulrike Mäder, Jörg Stülke, Erhard Bremer, Uwe Völker, Christoph Wittmann
Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective.
Environ Microbiol: 2014, 16(6);1898-917
[PubMed:24571712] [WorldCat.org] [DOI] (I p)

Yun Xia Duan, Tao Chen, Xun Chen, Xue Ming Zhao
Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 85(6);1907-14
[PubMed:19779711] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

P Rowland, A K Basak, S Gover, H R Levy, M J Adams
The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A resolution.
Structure: 1994, 2(11);1073-87
[PubMed:7881907] [WorldCat.org] [DOI] (P p)

S Ujita, K Kimura
Glucose-6-phosphate dehydrogenase, vegetative and spore Bacillus subtilis.
Methods Enzymol: 1982, 89 Pt D;258-61
[PubMed:6292660] [WorldCat.org] [DOI] (P p)