Zwf

• Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
  
  

Gene name	zwf
Synonyms	yqjJ
Essential	no
Product	glucose 6-phosphate dehydrogenase
Function	initiation of the pentose phosphate pathway
Gene expression levels in SubtiExpress: zwf
Metabolic function and regulation of this protein in SubtiPathways: zwf
MW, pI	55 kDa, 5.28
Gene length, protein length	1467 bp, 489 aa
Immediate neighbours	rnz, gndA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

The gene

Basic information

• Locus tag: BSU23850

Database entries

• BsubCyc: BSU23850

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)

• Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • Two Substrate Michaelis-Menten PubMed
  • specific activity: 0.375 µmol min^-1 (mg protein)^-1 PubMed

• Domains:

• Modification:

• Cofactors: Mg2+, Mn2+, Ca2+

• Effectors of protein activity:
  • NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23850

• Structure: 1DPG (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed

• UniProt: P54547

• KEGG entry: [3]

• E.C. number: 1.1.1.49

Additional information

The enzyme is a dimer PubMed

Expression and regulation

• Operon: zwf PubMed

• Expression browser: zwf PubMed

• Sigma factor:

• Regulation: constitutive PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2681 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3601 PubMed

Biological materials

• Mutant:
  • SM-NB3 (zwf-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1401 (spc, based on pGP1331), available in the Stülke lab