UppS

• Description: undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis
  
  

Gene name	uppS
Synonyms	yluA
Essential	yes PubMed
Product	undecaprenyl pyrophosphate synthetase
Function	cell wall biosynthesis
Gene expression levels in SubtiExpress: uppS
Metabolic function and regulation of this protein in SubtiPathways: UppS
MW, pI	29 kDa, 5.408
Gene length, protein length	780 bp, 260 aa
Immediate neighbours	frr, cdsA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of lipids, essential genes

The gene

Basic information

• Locus tag: BSU16530

Phenotypes of a mutant

• essential PubMed
• uppS is dispensable in wall-less L-forms PubMed
• the uppS86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin PubMed

Database entries

• BsubCyc: BSU16530

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate

• Protein family: UPP synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU16530

• Structure: 1X09 (the protein from E. coli, 49% identity, 77% similarity) PubMed

• UniProt: O31751

• KEGG entry: [2]

• E.C. number: 2.5.1.31

Expression and regulation

• Operon:

• Expression browser: uppS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yoshikazu Kawai, Romain Mercier, Jeff Errington
Bacterial cell morphogenesis does not require a preexisting template structure.
Curr Biol: 2014, 24(8);863-7
[PubMed:24704074] [WorldCat.org] [DOI] (I p)

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Yong Heon Lee, John D Helmann
Reducing the Level of Undecaprenyl Pyrophosphate Synthase Has Complex Effects on Susceptibility to Cell Wall Antibiotics.
Antimicrob Agents Chemother: 2013, 57(9);4267-4275
[PubMed:23796923] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Kozo Ochi
Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements.
J Bacteriol: 2012, 194(20);5632-7
[PubMed:22904278] [WorldCat.org] [DOI] (I p)

Rey-Ting Guo, Tzu-Ping Ko, Annie P-C Chen, Chih-Jung Kuo, Andrew H-J Wang, Po-Huang Liang
Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis.
J Biol Chem: 2005, 280(21);20762-74
[PubMed:15788389] [WorldCat.org] [DOI] (P p)