PonA

• Description: class A penicillin-binding protein 1A/1B, contributes to cell elongation and cell division
  
  

Gene name	ponA
Synonyms
Essential	no
Product	penicillin-binding protein 1A/1B
Function	bifunctional glucosyltransferase/ transpeptidase
Gene expression levels in SubtiExpress: ponA
Interactions involving this protein in SubtInteract: PonA
Metabolic function and regulation of this protein in SubtiPathways: PonA
MW, pI	99 kDa, 4.752
Gene length, protein length	2742 bp, 914 aa
Immediate neighbours	recU, ypoC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU22320

Phenotypes of a mutant

• prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed
• deletion of ponA restores growth and normal shape of a yvcK mutant on gluconeogenic carbon sources PubMed

Database entries

• BsubCyc: BSU22320

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • EzrA-PonA PubMed
  • GpsB-PonA PubMed
  • MreB-PonA PubMed

• Localization:
  • membrane associated PubMed
  • localizes to the spore septum during sporulation PubMed
  • localizes to the vegetative septum PubMed, this depends on YvcK PubMed

Database entries

• BsubCyc: BSU22320

• Structure:

• UniProt: P39793

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recU-ponA PubMed

• Expression browser: ponA PubMed

• Sigma factor: SigA PubMed, SigM PubMed

• Regulation: constitutive during vegetative growth PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

References

Reviews

Johann Mignolet, Patrick H Viollier
A sweet twist gets Bacillus into shape.
Mol Microbiol: 2011, 80(2);283-5
[PubMed:21371139] [WorldCat.org] [DOI] (I p)