NrgA

• Description: ammonium transporter, required at low ammonium concentration
  
  

Gene name	nrgA
Synonyms	amtB
Essential	no
Product	ammonium transporter
Function	ammonium uptake
Gene expression levels in SubtiExpress: nrgA
Interactions involving this protein in SubtInteract: NrgA
Metabolic function and regulation of this protein in SubtiPathways: nrgA
MW, pI	42 kDa, 5.727
Gene length, protein length	1212 bp, 404 aa
Immediate neighbours	ywoB, nrgB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, glutamate metabolism, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU36510

Database entries

• BsubCyc: BSU36510

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NrgA-NrgB PubMed

• Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

• BsubCyc: BSU36510

• Structure: 2NUU (the complex with NrgB, from E. coli) PubMed

• UniProt: Q07429

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB PubMed

• Expression browser: nrgA PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by ammonium limitation
  • induced by ammonium limitation (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP739 (cat), GP255 (nrgAB, cat), both available in the Stülke lab
  • 1A920 ( nrgA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion: pGP168 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Reviews

Arnaud Javelle, Domenico Lupo, Xiao-Dan Li, Mike Merrick, Mohamed Chami, Pierre Ripoche, Fritz K Winkler
Structural and mechanistic aspects of Amt/Rh proteins.
J Struct Biol: 2007, 158(3);472-81
[PubMed:17368911] [WorldCat.org] [DOI] (P p)

G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624] [WorldCat.org] [DOI] (P p)