capB

capB
168

part of the CapB-CapC glutamic acid ligase, capsular polyglutamate biosynthesis

Locus
BSU_35900
Molecular weight
43.79 kDa
Isoelectric point
5.07
Protein length
393 aaSequenceBlast
Gene length
1182 bpSequenceBlast
Function
capsule synthesis
Product
poly-gamma-glutamate synthetase
Essential
no
Synonyms
capB, ywsC, pgsB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0771 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,699,446  3,700,627
Phenotypes of a mutant
no synthesis of the poly-gamma-glutamate capsule PubMed
The protein
Catalyzed reaction/ biological activity
synthesis of poly-gamma-glutamate from L-Glu PubMed
Structure
P96736 (AlphaFold)
Expression and Regulation
Operons
Genes
capB-capC-capA-capE
Description
PubMed
Regulatory mechanism
DegU: activation, DegU-P, PubMed, in degU regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
expression of the operon is increased in 'motA' or 'motB' mutants due to increased DegU phosphorylation PubMed
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capB→capE

2025-03-16 08:50:33

ghost

109

bf6b0bf03353d832d0fb99e09c0c2fcd8af8f057

1302D3190C59C849B9803B3A11C008FA08B67E54

Biological materials
Mutant
MGNA-B262 (ywsC::erm), available at the NBRP B. subtilis, Japan
BKE35900 (capB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTTTTCGACATCTCC,  downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA
BKK35900 (capB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTTTTCGACATCTCC,  downstream forward: _UP4_GTAAGCTAGGGGGAAATGCA
References
Reviews
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Original Publications
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86C05126ADBA22BB1771B1FA3D214B2E7A36311E

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Time of last update: 2025-04-08 12:07:07

Author of last update: Jstuelk