AzoR1

• Description: azoreductase, involved in quinone detoxification
  
  

Gene name	azoR1
Synonyms	yocJ
Essential	no
Product	azoreductase
Function	quinone detoxification
Gene expression levels in SubtiExpress: azoR1
MW, pI	22 kDa, 4.994
Gene length, protein length	624 bp, 208 aa
Immediate neighbours	bsrB, yocK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress, most abundant proteins

This gene is a member of the following regulons

YodB regulon

The gene

Basic information

• Locus tag: BSU19230

Database entries

• BsubCyc: BSU19230

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): AzoR2

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU19230

• Structure: 3P0R (from B. anthracis, 64% identity, 81% similarity)

• UniProt: O35022

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: azoR1 PubMed

• Sigma factor:

• Regulation:
  • induced by thiol-reactive compounds (catechol, menaquinone) (YodB) PubMed

• Regulatory mechanism:
  • YodB: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Montira Leelakriangsak, Nguyen Thi Thu Huyen, Stefanie Töwe, Nguyen van Duy, Dörte Becher, Michael Hecker, Haike Antelmann, Peter Zuber
Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis.
Mol Microbiol: 2008, 67(5);1108-24
[PubMed:18208493] [WorldCat.org] [DOI] (I p)

Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann
The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis.
Mol Microbiol: 2007, 66(1);40-54
[PubMed:17725564] [WorldCat.org] [DOI] (P p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)