SpoVS

• Description: required for dehydratation of the spore core and assembly of the coat, mutation increases SigD-dependent gene expression, might act via SinR
  
  

Gene name	spoVS
Synonyms
Essential	no
Product	unknown
Function	spore coat assembly, spore core dehydratation
Gene expression levels in SubtiExpress: spoVS
MW, pI	8 kDa, 6.475
Gene length, protein length	258 bp, 86 aa
Immediate neighbours	ymdB, tdh
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation/ other

This gene is a member of the following regulons

SigH regulon

The gene

Basic information

• Locus tag: BSU16980

Phenotypes of a mutant

increased SigD-dependent gene expression, this might occur via SinR PubMed

Database entries

• BsubCyc: BSU16980

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU16980

• Structure: 2EK0 (from Thermus thermophilus, 56% identity)

• UniProt: P45693

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoVS PubMed

• Expression browser: spoVS PubMed

• Sigma factor: SigH PubMed

• Regulation:
  • expressed at the onset of stationary phase (SigH) PubMed

• Regulatory mechanism:

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

• Mutant: GP1601 (cat), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody: