InfB

• Description: translation initiation factor IF-2
  
  

Gene name	infB
Synonyms
Essential	yes PubMed
Product	initiation factor IF-2
Function	translation
Gene expression levels in SubtiExpress: infB
MW, pI	78 kDa, 5.191
Gene length, protein length	2148 bp, 716 aa
Immediate neighbours	ylxQ, ylxP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16630

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU16630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Initiation of translation

• Protein family: IF-2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: Nucleotide-binding domain that competitively binds GTP or ppGpp PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity: GTP and ppGpp PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16630

• Structure: 1D1N (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), 1Z9B (C1-subdomain, Geobacillus stearothermophilus)

• UniProt: P17889

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylxS-nusA-ylxR-ylxQ-infB-ylxP-rbfA PubMed

• Expression browser: infB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • Interaction with ppGpp interferes with IF2-dependent initiation complex formation, severely inhibits initiation dipeptide formation, and blocks the initiation step of translation.PubMed

• Regulatory mechanism:

• Additional information: Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: