LigA

• Description: DNA ligase (NAD-dependent)
  
  

Gene name	ligA
Synonyms	lig, yerG
Essential	yes PubMed
Product	DNA ligase (NAD-dependent)
Function	replication
Gene expression levels in SubtiExpress: ligA
MW, pI	74 kDa, 4.708
Gene length, protein length	2004 bp, 668 aa
Immediate neighbours	pcrA, yerH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU06620

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU06620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NAD⁺ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)

• Protein family: BRCT domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU06620

• Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)

• UniProt: O31498

• KEGG entry: [3]

• E.C. number: 6.5.1.2

Expression and regulation

• Operon: pcrB-pcrA-ligA-yerH PubMed

• Expression browser: ligA PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: