NdoAI

• Description: antitoxin, antagonist for EndoA
  
  

Gene name	ndoAI
Synonyms	ydcD, mazE
Essential	no
Product	antitoxin
Function	inhibition of NdoA
Gene expression levels in SubtiExpress: ndoAI
Interactions involving this protein in SubtInteract: NdoAI
MW, pI	10 kDa, 6.138
Gene length, protein length	279 bp, 93 aa
Immediate neighbours	alr, ndoA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins

The gene

Basic information

• Locus tag: BSU04650

Database entries

• BsubCyc: BSU04650

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mazE/ndoAI family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NdoA(2)-NdoAI(2)-NdoA(2) PubMed

• Localization:

Database entries

• BsubCyc: BSU04650

• Structure:
  • 4ME7 (NdoA-NdoAI complex) PubMed

• UniProt: P96621

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ndoAI-ndoA PubMed

• Expression browser: ndoAI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dhirendra K Simanshu, Yoshihiro Yamaguchi, Jung-Ho Park, Masayori Inouye, Dinshaw J Patel
Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis.
Mol Cell: 2013, 52(3);447-58
[PubMed:24120662] [WorldCat.org] [DOI] (I p)

Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361] [WorldCat.org] [DOI] (P p)

Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409] [WorldCat.org] [DOI] (P p)