PutB

• Description: proline dehydrogenase (L-proline, NAD)
  
  

Gene name	putB
Synonyms	ycgM
Essential	no
Product	proline dehydrogenase (L-proline, NAD)
Function	proline utilization
Gene expression levels in SubtiExpress: putB
Metabolic function and regulation of this protein in SubtiPathways: putB
MW, pI	34 kDa, 7.291
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	ycgL, putC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU03200

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• BsubCyc: BSU03200

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)

• Protein family: proline dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): FadM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03200

• Structure:

• UniProt: P94390

• KEGG entry: [3]

• E.C. number: 1.5.99.8

Expression and regulation

• Operon: putB-putC-putP 22139509 PubMed

• Expression browser: putB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed
  • Spo0A: transcription activation PubMed
  • CodY: transcription repression, displacement of PutR PubMed

• Additional information:
  • overexpressed in a pnpA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: