NarH

• Description: nitrate reductase (beta subunit)
  
  

Gene name	narH
Synonyms
Essential	no
Product	nitrate reductase (beta subunit)
Function	nitrate respiration, nitrogen assimilation
Gene expression levels in SubtiExpress: narH
Interactions involving this protein in SubtInteract: NarH
MW, pI	55 kDa, 5.108
Gene length, protein length	1461 bp, 487 aa
Immediate neighbours	narJ, narG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

Fnr regulon

The gene

Basic information

• Locus tag: BSU37270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • Fe-S cluster PubMed

• Effectors of protein activity:

• Interactions:
  • NarG-NarH PubMed
  • NarH-NarI PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P42176

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: narG-narH-narJ-narI PubMed

• Expression browser: narH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • Fnr: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)