MurAA
- Description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Gene name | murAA |
Synonyms | murA |
Essential | yes PubMed |
Product | UDP-N-acetylglucosamine 1-carboxyvinyltransferase |
Function | peptidoglycan precursor biosynthesis |
Gene expression levels in SubtiExpress: murAA | |
Metabolic function and regulation of this protein in SubtiPathways: murAA | |
MW, pI | 46 kDa, 5.45 |
Gene length, protein length | 1308 bp, 436 aa |
Immediate neighbours | spoIID, ywmB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36760
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)
- Protein family: MurA subfamily (according to Swiss-Prot)
- Paralogous protein(s): MurAB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 3SG1 (from B. anthracis, 79% identity, 94% similarity)
- UniProt: P70965
- KEGG entry: [3]
- E.C. number: 2.5.1.7
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- pRSETA-murAA, Expression of His(6)-MurAA, avalable in Ulf Gerth's lab PubMed
- pGP2597: (IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Ankur Gautam, Praveen Rishi, Rupinder Tewari
UDP-N-acetylglucosamine enolpyruvyl transferase as a potential target for antibacterial chemotherapy: recent developments.
Appl Microbiol Biotechnol: 2011, 92(2);211-25
[PubMed:21822642]
[WorldCat.org]
[DOI]
(I p)
Original publications