SpoIIID

• Description: transcriptional regulator (repressor or activator) of a subset of sigma-E-dependent genes
  
  

Gene name	spoIIID
Synonyms
Essential	no
Product	transcriptional regulator
Function	regulation of mother cell gene expression
Gene expression levels in SubtiExpress: spoIIID
Metabolic function and regulation of this protein in SubtiPathways: spoIIID
MW, pI	10 kDa, 9.496
Gene length, protein length	279 bp, 93 aa
Immediate neighbours	mbl, usd
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU36420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds its DNA targets as a monomeric protein (possible due to the two DNA-binding motifs) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2L0K (complex with DNA)

• UniProt: P15281

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: usd-spoIIID-mbl-flhO-flhP PubMed

• Expression browser: spoIIID PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bin Chen, Paul Himes, Yu Liu, Yang Zhang, Zhenwei Lu, Aizhuo Liu, Honggao Yan, Lee Kroos
Structure of bacterial transcription factor SpoIIID and evidence for a novel mode of DNA binding.
J Bacteriol: 2014, 196(12);2131-42
[PubMed:24584501] [WorldCat.org] [DOI] (I p)

Sarah E Ebmeier, Irene S Tan, Katie Rose Clapham, Kumaran S Ramamurthi
Small proteins link coat and cortex assembly during sporulation in Bacillus subtilis.
Mol Microbiol: 2012, 84(4);682-96
[PubMed:22463703] [WorldCat.org] [DOI] (I p)

Paul Himes, Steven J McBryant, Lee Kroos
Two regions of Bacillus subtilis transcription factor SpoIIID allow a monomer to bind DNA.
J Bacteriol: 2010, 192(6);1596-606
[PubMed:20061473] [WorldCat.org] [DOI] (I p)

Lijuan Wang, John Perpich, Adam Driks, Lee Kroos
One perturbation of the mother cell gene regulatory network suppresses the effects of another during sporulation of Bacillus subtilis.
J Bacteriol: 2007, 189(23);8467-73
[PubMed:17890309] [WorldCat.org] [DOI] (I p)

Chris Guillot, Charles P Moran
Essential internal promoter in the spoIIIA locus of Bacillus subtilis.
J Bacteriol: 2007, 189(20);7181-9
[PubMed:17693505] [WorldCat.org] [DOI] (P p)

Lijuan Wang, John Perpich, Adam Driks, Lee Kroos
Maintaining the transcription factor SpoIIID level late during sporulation causes spore defects in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7302-9
[PubMed:17693499] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

H Ichikawa, L Kroos
Combined action of two transcription factors regulates genes encoding spore coat proteins of Bacillus subtilis.
J Biol Chem: 2000, 275(18);13849-55
[PubMed:10788508] [WorldCat.org] [DOI] (P p)

A Decatur, M T McMurry, B N Kunkel, R Losick
Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame.
J Bacteriol: 1997, 179(4);1324-8
[PubMed:9023218] [WorldCat.org] [DOI] (P p)

B Zhang, R A Daniel, J Errington, L Kroos
Bacillus subtilis SpoIIID protein binds to two sites in the spoVD promoter and represses transcription by sigmaE RNA polymerase.
J Bacteriol: 1997, 179(3);972-5
[PubMed:9006059] [WorldCat.org] [DOI] (P p)

R Halberg, V Oke, L Kroos
Effects of Bacillus subtilis sporulation regulatory protein SpoIIID on transcription by sigma K RNA polymerase in vivo and in vitro.
J Bacteriol: 1995, 177(7);1888-91
[PubMed:7896717] [WorldCat.org] [DOI] (P p)

Y Abhayawardhane, G C Stewart
Bacillus subtilis possesses a second determinant with extensive sequence similarity to the Escherichia coli mreB morphogene.
J Bacteriol: 1995, 177(3);765-73
[PubMed:7836311] [WorldCat.org] [DOI] (P p)

R Halberg, L Kroos
Sporulation regulatory protein SpoIIID from Bacillus subtilis activates and represses transcription by both mother-cell-specific forms of RNA polymerase.
J Mol Biol: 1994, 243(3);425-36
[PubMed:7966271] [WorldCat.org] [DOI] (P p)

P A Levin, N Fan, E Ricca, A Driks, R Losick, S Cutting
An unusually small gene required for sporulation by Bacillus subtilis.
Mol Microbiol: 1993, 9(4);761-71
[PubMed:8231808] [WorldCat.org] [DOI] (P p)

R Halberg, L Kroos
Fate of the SpoIIID switch protein during Bacillus subtilis sporulation depends on the mother-cell sigma factor, sigma K.
J Mol Biol: 1992, 228(3);840-9
[PubMed:1469717] [WorldCat.org] [DOI] (P p)

L Zheng, R Halberg, S Roels, H Ichikawa, L Kroos, R Losick
Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes.
J Mol Biol: 1992, 226(4);1037-50
[PubMed:1518043] [WorldCat.org] [DOI] (P p)

K M Tatti, C H Jones, C P Moran
Genetic evidence for interaction of sigma E with the spoIIID promoter in Bacillus subtilis.
J Bacteriol: 1991, 173(24);7828-33
[PubMed:1744038] [WorldCat.org] [DOI] (P p)

S Cutting, A Driks, R Schmidt, B Kunkel, R Losick
Forespore-specific transcription of a gene in the signal transduction pathway that governs Pro-sigma K processing in Bacillus subtilis.
Genes Dev: 1991, 5(3);456-66
[PubMed:1900494] [WorldCat.org] [DOI] (P p)

L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789] [WorldCat.org] [DOI] (P p)

C M Stevens, J Errington
Differential gene expression during sporulation in Bacillus subtilis: structure and regulation of the spoIIID gene.
Mol Microbiol: 1990, 4(4);543-51
[PubMed:2112673] [WorldCat.org] [DOI] (P p)

B Kunkel, L Kroos, H Poth, P Youngman, R Losick
Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis.
Genes Dev: 1989, 3(11);1735-44
[PubMed:2514119] [WorldCat.org] [DOI] (P p)