SpeA

• Description: arginine decarboxylase, required for biofilm formation
  
  

Gene name	speA
Synonyms	cad
Essential	no
Product	arginine decarboxylase
Function	spermidine, polyamine biosynthesis
Gene expression levels in SubtiExpress: speA
Metabolic function and regulation of this protein in SubtiPathways: speA
MW, pI	53 kDa, 5.081
Gene length, protein length	1470 bp, 490 aa
Immediate neighbours	sr1, yktA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biofilm formation

The gene

Basic information

• Locus tag: BSU14630

Phenotypes of a mutant

• no biofilm formation PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-arginine = agmatine + CO₂ (according to Swiss-Prot)

• Protein family: Orn/Lys/Arg decarboxylase class-I family (according to Swiss-Prot)

• Paralogous protein(s): YaaO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P21885

• KEGG entry: [2]

• E.C. number: 4.1.1.19

Expression and regulation

• Operon:

• Expression browser: speA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Laura Hobley, Sok Ho Kim, Yukari Maezato, Susan Wyllie, Alan H Fairlamb, Nicola R Stanley-Wall, Anthony J Michael
Norspermidine is not a self-produced trigger for biofilm disassembly.
Cell: 2014, 156(4);844-54
[PubMed:24529384] [WorldCat.org] [DOI] (I p)

Matthew Burrell, Colin C Hanfrey, Ewan J Murray, Nicola R Stanley-Wall, Anthony J Michael
Evolution and multiplicity of arginine decarboxylases in polyamine biosynthesis and essential role in Bacillus subtilis biofilm formation.
J Biol Chem: 2010, 285(50);39224-38
[PubMed:20876533] [WorldCat.org] [DOI] (I p)

Xiao Yan Liu, Jian Lei, Xiang Liu, Xiao Dong Su, Lanfen Li
Preliminary X-ray crystallographic studies of Bacillus subtilis SpeA protein.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 3);282-4
[PubMed:19255484] [WorldCat.org] [DOI] (I p)

A Sekowska, P Bertin, A Danchin
Characterization of polyamine synthesis pathway in Bacillus subtilis 168.
Mol Microbiol: 1998, 29(3);851-8
[PubMed:9723923] [WorldCat.org] [DOI] (P p)