NagZ

• Description: N-acetylglucosaminidase
  
  

Gene name	nagZ
Synonyms	yzbA, ybbD
Essential	no
Product	N-acetylglucosaminidase
Function	cell wall recycling
Gene expression levels in SubtiExpress: nagZ
Metabolic function and regulation of this protein in SubtiPathways: nagZ
MW, pI	70 kDa, 9.76
Gene length, protein length	1926 bp, 642 aa
Immediate neighbours	ybbC, amiE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

The gene

Basic information

• Locus tag: BSU01660

Phenotypes of a mutant

increased autolysis PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cleaves muropeptides derived from peptidoglycan, but not peptidoglycan itself PubMed

• Protein family: glycosyl hydrolase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (with signal peptide), remains to some extent cell wall-associated PubMed

Database entries

• Structure: 3BMX PubMed

• UniProt: P40406

• KEGG entry: [2]

• E.C. number: 3.2.1.52

Additional information

The gene is mis-annotated in KEGG as an ortholog of beta-N-acetylhexosaminidase EC 3.2.1.52. It is marked in MetaCyc as “similar to beta-hexosaminidase”. No EC annotation is available in Swiss-ProtSwiss-Prot.supporting the annotation is available. PubMed

Expression and regulation

• Operon: murQ-murR-murP-amiE-nagZ-ybbC PubMed

• Expression browser: nagZ PubMed

• Sigma factor:

• Regulation:
  • expressed in late exponential and early stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: available in Christoph Mayer's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional references: PubMed