RecU

• Description: DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU
  
  

Gene name	recU
Synonyms	recG, prfA, yppB, jopB
Essential	no
Product	Holliday junction resolvase
Function	DNA repair, homologous recombination and chromosome segregation
Gene expression levels in SubtiExpress: recU
Interactions involving this protein in SubtInteract: RecU
MW, pI	23 kDa, 9.511
Gene length, protein length	618 bp, 206 aa
Immediate neighbours	yppC, ponA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU22310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • form dimers PubMed
  • RecA-RecU PubMed
  • RecU-RuvB PubMed

• Localization:

Database entries

• Structure: 1ZP7

• UniProt: P39792

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recU-ponA PubMed

• Expression browser: recU PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP891 (recU::cat), available in Jörg Stülke's lab
  • 1A895 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

S C West
Processing of recombination intermediates by the RuvABC proteins.
Annu Rev Genet: 1997, 31;213-44
[PubMed:9442895] [WorldCat.org] [DOI] (P p)

RecU in other organisms

Ana R Pereira, Patricia Reed, Helena Veiga, Mariana G Pinho
The Holliday junction resolvase RecU is required for chromosome segregation and DNA damage repair in Staphylococcus aureus.
BMC Microbiol: 2013, 13;18
[PubMed:23356868] [WorldCat.org] [DOI] (I e)