Mdh
- Description: malate dehydrogenase
Gene name | mdh |
Synonyms | citH |
Essential | no |
Product | malate dehydrogenase |
Function | TCA cycle |
Gene expression levels in SubtiExpress: mdh | |
Interactions involving this protein in SubtInteract: Mdh | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 33 kDa, 4.727 |
Gene length, protein length | 936 bp, 312 aa |
Immediate neighbours | phoP, icd |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, membrane proteins, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = oxaloacetate + NADH (according to Swiss-Prot)
- Protein family: MDH type 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- Cofactor(s): NAD+
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot), membrane associated PubMed
Database entries
- Structure: 1EMD (E.coli)
- UniProt: P49814
- KEGG entry: [3]
- E.C. number: 1.1.1.37
Additional information
- The enzyme is a tetramer PubMed
- extensive information on the structure and enzymatic properties of Mdh can be found at Proteopedia
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP719(spc) & GP1150(spc), available in Jörg Stülke's lab
- GP790 (citZ-icd-mdh::kan), available in Jörg Stülke's lab
- Expression vector:
- pGP385: for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
- pGP1123 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
- pGP1755 (expression / purification of Mdh-S149A, with N-terminal His-tag from E. coli, in pWH844), available in Jörg Stülke's lab
- pGP1764 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)
- GP1438(mdh-Strep (spc)) & GP1440(mdh-Strep (cat)), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion: GP1431 (spc, based on pGP1870), available in Jörg Stülke's lab
- YFP fusion: GP1429 (spc, based on pGP1871), available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
- FLAG-tag construct:
- GP1130 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References