Sandbox

• Description: glutamine-fructose-6-phosphate transaminase

Murein recycling |- |style="background:#ABCDEF;" align="center"| MW, pI || 65 kDa, 4.796 |- |style="background:#ABCDEF;" align="center"| Gene length, protein length || 1800 bp, 600 aa |- |style="background:#ABCDEF;" align="center"|Immediate neighbours || glmM, ybbU |- |colspan="2" style="background:#FAF8CC;" align="center"|Get the DNA and protein [EMBLCDS:CAB11954+-newId sequences] (Barbe et al., 2009) |- |colspan="2" | Genetic context This image was kindly provided by SubtiList |- |} = Categories containing this gene/protein = cell wall synthesis, biosynthesis of cell wall components, essential genes = This gene is a member of the following regulons = glmS ribozyme =The gene= === Basic information === * Locus tag: BSU01780 ===Phenotypes of a mutant === essential PubMed === Database entries === * DBTBS entry: no entry * SubtiList entry:[1] === Additional information=== =The protein= === Basic information/ Evolution === * Catalyzed reaction/ biological activity: L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) * Protein family: * Paralogous protein(s): === Extended information on the protein === * Kinetic information: * Domains: * Modification: * Cofactor(s): * Effectors of protein activity: * Interactions: * Localization: ** cytoplasm (according to Swiss-Prot) === Database entries === * Structure:HIV (from Bacillus subtilis, 100% identity) PubMed2VF4 (GlmS from E. coli, 39% identity, 58% similarity) PubMed ** the ribozyme: 3G8S, 3G9C, 3G8T, 3G95, 3G96 (all for the ribozyme from Bacillus anthracis), 2HO7 (the ribozyme from Thermonanaerobacter tengcongensis) * UniProt:P39754 * KEGG entry:[2] * E.C. number:2.6.1.16 === Additional information=== :* subject to Clp-dependent proteolysis upon glucose starvation PubMed =Expression and regulation= * Operon:ybbP-ybbR-glmM-glmS * Expression browser:glmSPubMed * Sigma factor:SigAPubMed * Regulation: ** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine PubMed ** glmS is only expressed in the absence of glucosamine 6-phosphate (glmSribozyme) * Regulatory mechanism:glmSribozyme: glucosamine 6-phosphate binds the leader mRNA, and a riboswitch with ribozyme activity cleaves off the glmS section from the mRNA, resulting in stopp of transcript elongation * Additional information: ** subject to Clp-dependent proteolysis upon glucose starvation PubMed ** A ncRNA is predicted between glmM and glmSPubMed =Biological materials = * Mutant: * Expression vector: * lacZ fusion: * GFP fusion: * two-hybrid system: * Antibody: =Labs working on this gene/protein= Wade Winkler, University of Texas, USA, Homepage =Your additional remarks= =References= ==Reviews== 18279655 ==The glmS Ribozyme== 18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 ==Other Original Publications== Additional publications:PubMed 14343123 17981983 ,11160890, 18295797 20525796