DnaI

• Description: primosome component (helicase loader), part of the replisome
  
  

Gene name	dnaI
Synonyms	ytxA, dnaY
Essential	yes PubMed
Product	primosome component (helicase loader)
Function	DNA replication
Gene expression levels in SubtiExpress: dnaI
Interactions involving this protein in SubtInteract: DnaI
MW, pI	35 kDa, 5.743
Gene length, protein length	933 bp, 311 aa
Immediate neighbours	ytxB, dnaB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU28980

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-DnaI PubMed, DnaC-DnaI PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization: cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed forms foci, close to oriC PubMed

Database entries

• Structure:
  • 2K7R (N-terminal domain)
  • 4M4W (the DnaC-DnaI-DnaG complex) PubMed

• UniProt: P06567

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dnaI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)

Original publications

Bin Liu, William K Eliason, Thomas A Steitz
Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly.
Nat Commun: 2013, 4;2495
[PubMed:24048025] [WorldCat.org] [DOI] (I p)

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

Wiep Klaas Smits, Alexi I Goranov, Alan D Grossman
Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
Mol Microbiol: 2010, 75(2);452-61
[PubMed:19968790] [WorldCat.org] [DOI] (I p)

Kuang-Lei Tsai, Yu-Hua Lo, Yuh-Ju Sun, Chwan-Deng Hsiao
Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.
J Mol Biol: 2009, 393(5);1056-69
[PubMed:19744498] [WorldCat.org] [DOI] (I p)

Karin V Loscha, Kristaps Jaudzems, Charikleia Ioannou, Xun-Cheng Su, Flynn R Hill, Gottfried Otting, Nicholas E Dixon, Edvards Liepinsh
A novel zinc-binding fold in the helicase interaction domain of the Bacillus subtilis DnaI helicase loader.
Nucleic Acids Res: 2009, 37(7);2395-404
[PubMed:19255093] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Marion Velten, Stephen McGovern, Stéphanie Marsin, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
A two-protein strategy for the functional loading of a cellular replicative DNA helicase.
Mol Cell: 2003, 11(4);1009-20
[PubMed:12718886] [WorldCat.org] [DOI] (P p)

P Soultanas
A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus.
Nucleic Acids Res: 2002, 30(4);966-74
[PubMed:11842108] [WorldCat.org] [DOI] (I p)

C Bruand, M Farache, S McGovern, S D Ehrlich, P Polard
DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome.
Mol Microbiol: 2001, 42(1);245-55
[PubMed:11679082] [WorldCat.org] [DOI] (P p)

Y Imai, N Ogasawara, D Ishigo-Oka, R Kadoya, T Daito, S Moriya
Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids.
Mol Microbiol: 2000, 36(5);1037-48
[PubMed:10844689] [WorldCat.org] [DOI] (P p)