EcsA

• Description: ABC transporter (ATP-binding protein), mutation affects proteolysis by RasP (no processing of RsiW and FtsL)
  
  

Gene name	ecsA
Synonyms	prsT, ecs-26, yhaD
Essential	no
Product	ABC transporter (ATP-binding protein)
Function	regulation of the secretion apparatus and of intra-membrane proteolysis
Gene expression levels in SubtiExpress: ecsA
Interactions involving this protein in SubtInteract: EcsA
MW, pI	27 kDa, 5.635
Gene length, protein length	741 bp, 247 aa
Immediate neighbours	hit, ecsB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, protein secretion, sigma factors and their control, membrane proteins

The gene

Basic information

• Locus tag: BSU10040

Phenotypes of a mutant

no processing of RsiW and FtsL PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transporter domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: EcsA-EcsB PubMed

• Localization: membrane associated (via EcsB) PubMed

Database entries

• Structure:

• UniProt: P55339

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ecsA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Janine Heinrich, Tuula Lundén, Vesa P Kontinen, Thomas Wiegert
The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP.
Microbiology (Reading): 2008, 154(Pt 7);1989-1997
[PubMed:18599827] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

S Leskelä, E Wahlström, H L Hyyryläinen, M Jacobs, A Palva, M Sarvas, V P Kontinen
Ecs, an ABC transporter of Bacillus subtilis: dual signal transduction functions affecting expression of secreted proteins as well as their secretion.
Mol Microbiol: 1999, 31(2);533-43
[PubMed:10027970] [WorldCat.org] [DOI] (P p)

Soile Leskelä, Vesa P Kontinen, Matti Sarvas
Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence.
Microbiology (Reading): 1996, 142 ( Pt 1);71-77
[PubMed:8581172] [WorldCat.org] [DOI] (P p)