Sandbox

• Description: inhibition of the pro-sigma-K processing machinery
  
  

Gene name	bofA
Synonyms
Essential	no
Product	inhibitor of pro-SigK processing
Function	control of SigK activation
Gene expression levels in SubtiExpress: bofA
Interactions involving this protein in SubtInteract: BofA
MW, pI	8 kDa, 9.32
Gene length, protein length	261 bp, 87 aa
Immediate neighbours	yaaL, rrnA-16S
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU00230

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • BofA-SpoIVB PubMed
  • SpoIVFB-BofA, to inhibit proteolytic activity of SpoIVFB PubMed
  • SpoIVFA-BofA, recruitment of BofA to SpoIVFB PubMed

• Localization:
  • forespore outer membrane (according to Swiss-Prot)
  • integral membrane protein PubMed

Database entries

• Structure:

• UniProt: P24282

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: bofA PubMed

• Expression browser: bofA PubMed

• Sigma factor: SigE PubMed

• Regulation: expressed during sporulation PubMed

• Regulatory mechanism: repressed by SpoIIID PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • 1S100 ( bofA::cat), PubMed, available at BGSC
  • 1S96 ( bofA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Simon Cutting, London, UK homepage