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• Description: tRNA specific adenosine deaminase
  
  

Gene name	yaaJ
Synonyms
Essential	no
Product	tRNA specific adenosine deaminase
Function	tRNA modification
Gene expression levels in SubtiExpress: yaaJ
MW, pI	17 kDa, 5.636
Gene length, protein length	483 bp, 161 aa
Immediate neighbours	yaaI, scr
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU00180

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)

• Paralogous protein(s):

• The corresponding gene in E. coli: EcoliWiki

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2B3J (the protein of Staphylococcus aureus, complex with RNA) PubMed

• UniProt: P21335

• KEGG entry: [3]

• E.C. number: 3.5.4.-

Expression and regulation

• Operon: yaaJ-scr

• Expression browser: yaaJ PubMed

• Sigma factor: SigA

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Heather C Losey, Alexander J Ruthenburg, Gregory L Verdine
Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA.
Nat Struct Mol Biol: 2006, 13(2);153-9
[PubMed:16415880] [WorldCat.org] [DOI] (P p)

Jeannette Wolf, André P Gerber, Walter Keller
tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli.
EMBO J: 2002, 21(14);3841-51
[PubMed:12110595] [WorldCat.org] [DOI] (P p)