Sandbox

• Description: seryl-tRNA-synthetase
  
  

Gene name	serS
Synonyms
Essential	yes PubMed
Product	seryl-tRNA-synthetase
Function	translation
Gene expression levels in SubtiExpress: serS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	48 kDa, 5.193
Gene length, protein length	1275 bp, 425 aa
Immediate neighbours	pdxT, trnSL-Ser1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU00130

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser) (according to Swiss-Prot)

• Protein family: Type-1 seryl-tRNA synthetase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1SES (from Thermus thermophilus, 36% identity, 56% similarity) PubMed

• UniProt: P37464

• KEGG entry: [3]

• E.C. number: 6.1.1.11

Expression and regulation

• Operon: serS PubMed

• Expression browser: serS PubMed

• Sigma factor:

• Regulation:
  • induced by serine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

C Condon, M Grunberg-Manago, H Putzer
Aminoacyl-tRNA synthetase gene regulation in Bacillus subtilis.
Biochimie: 1996, 78(6);381-9
[PubMed:8915527] [WorldCat.org] [DOI] (P p)

Biochimie 78: 381-389. PubMed