YvcL

• Description: WhiA family protein conserved and ubiquitous among gram-positive bacteria, putative transcription factor
  
  

Gene name	yvcL
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yvcL
MW, pI	36 kDa, 9.479
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	crh, yvcK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control

The gene

Basic information

• Locus tag: BSU34750

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: WhiA family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: suggested HTH domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3HYI (Crystal structure of full-length DUF199/WhiA from Thermotoga maritima), 3HYJ (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)

• UniProt: O06975

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Expression browser: yvcL PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Jörg Stülke's lab; plasmid pGP1468 was used to disrupt the gene.

• Expression vector: pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Your additional remarks

WhiA orthologues are present in all Gram-positive bacteria and best characterized by the WhiA sporulation control factor in Streptomyces coelicolor. They are thought to act as genetic regulators that have evolved from LAGLIDADG homing endonucleases (LHE).