PWH844
Revision as of 14:23, 8 October 2013 by Raphael2215 (talk | contribs)
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘
F Schirmer, S Ehrt, W Hillen
Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.
J Bacteriol: 1997, 179(4);1329-36
[PubMed:9023219]
[WorldCat.org]
[DOI]
(P p)
