YneA

• Description: inhibits cell division during SOS response
  
  

Gene name	yneA
Synonyms
Essential	no
Product	cell division control protein
Function	inhibits cell division during SOS response
Gene expression levels in SubtiExpress: yneA
MW, pI	11 kDa, 4.613
Gene length, protein length	315 bp, 105 aa
Immediate neighbours	lexA, yneB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU17860

Phenotypes of a mutant

• increased sensitivity to DNA-damaging agents PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: yneA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • LysM peptidoglycan binding domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted to the medium (signal peptide) PubMed

Database entries

• Structure:

• UniProt: Q45056

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yneA-yneB-ynzC PubMed

• Expression browser: yneA PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:
  • YneA is rapidly degraded by extracellular proteases PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Meike Buchholz, Hannes Nahrstedt, Mike H Pillukat, Veronika Deppe, Friedhelm Meinhardt
yneA mRNA instability is involved in temporary inhibition of cell division during the SOS response of Bacillus megaterium.
Microbiology (Reading): 2013, 159(Pt 8);1564-1574
[PubMed:23728628] [WorldCat.org] [DOI] (I p)

Allison H Mo, William F Burkholder
YneA, an SOS-induced inhibitor of cell division in Bacillus subtilis, is regulated posttranslationally and requires the transmembrane region for activity.
J Bacteriol: 2010, 192(12);3159-73
[PubMed:20400548] [WorldCat.org] [DOI] (I p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Yoshikazu Kawai, Shigeki Moriya, Naotake Ogasawara
Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis.
Mol Microbiol: 2003, 47(4);1113-22
[PubMed:12581363] [WorldCat.org] [DOI] (P p)