QdoR

• Description: transcriptional regulator (TetR family), control of qdoI-yxaH and lmrA-lmrB
  
  

Gene name	qdoR
Synonyms	yxaF
Essential	no
Product	transcriptional regulator
Function	control of quercetin utilization
Gene expression levels in SubtiExpress: qdoR
MW, pI	20 kDa, 6.111
Gene length, protein length	573 bp, 191 aa
Immediate neighbours	qdoI, yxnA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

LmrA regulon, QdoR regulon

The QdoR regulon:

• qdoI-yxaH, qdoR

The gene

Basic information

• Locus tag: BSU39990

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: TetR family

• Paralogous protein(s): LmrA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: flavonoids such as quercetin act as molecular inducers and result in release from DNA targets PubMed

• Interactions:

• Localization:

Database entries

• Structure: 1SGM

• UniProt: P42105

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: qdoR PubMed

• Expression browser: qdoR PubMed

• Sigma factor:

• Regulation:
  • induced by flavonoids such as quercetin (QdoR, LmrA) PubMed

• Regulatory mechanism:
  • LmrA: transcription repression PubMed
  • QdoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan

References

Original publications

Kazutake Hirooka, Yasutaro Fujita
Identification of aromatic residues critical to the DNA binding and ligand response of the Bacillus subtilis QdoR (YxaF) repressor antagonized by flavonoids.
Biosci Biotechnol Biochem: 2011, 75(7);1325-34
[PubMed:21737930] [WorldCat.org] [DOI] (I p)

Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
J Bacteriol: 2007, 189(14);5170-82
[PubMed:17483215] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)