GatC

• Description: production of glutamyl-tRNA(Gln)
  
  

Gene name	gatC
Synonyms	yerL, yedA
Essential	yes PubMed
Product	glutamyl-tRNA(Gln) amidotransferase (subunit C)
Function	translation
Gene expression levels in SubtiExpress: gatC
Interactions involving this protein in SubtInteract: GatC
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	10 kDa, 4.596
Gene length, protein length	288 bp, 96 aa
Immediate neighbours	opuE, gatA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU06670

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)

• Protein family: gatC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-3 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GatA-GatB-GatC
  • (GatA-GatB-GatC)-GltX-trnS-Gln (the "transamidosome") PubMed, see a model of the interaction PubMed

• Localization:

Database entries

• Structure: 2DF4 (GatA-GatB-GatC complex from Staphylococcus aureus) PubMed

• UniProt: O06492

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: gatC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

Akiyoshi Nakamura, Min Yao, Sarin Chimnaronk, Naoki Sakai, Isao Tanaka
Ammonia channel couples glutaminase with transamidase reactions in GatCAB.
Science: 2006, 312(5782);1954-8
[PubMed:16809541] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc Natl Acad Sci U S A: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)