MstX

• Description: membrane-integrating protein for membrane protein expression, MISTIC
  
  

Gene name	mstX
Synonyms
Essential	no
Product	MISTIC
Function	unknown
Gene expression levels in SubtiExpress: mstX
MW, pI	13 kDa, 4.549
Gene length, protein length	330 bp, 110 aa
Immediate neighbours	yuzI, yugO
Gene sequence (+200bp)	Protein sequence
Genetic context File:MstX context.gif
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, biofilm formation, membrane proteins

This gene is a member of the following regulons

SinR regulon

The gene

Basic information

• Locus tag: BSU31321

Phenotypes of a mutant

• reduced biofilm formation (can be suppressed by inactivation of sinR) PubMed
• overexpression result in increased biofilm formation even in a domesticated strain background PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: no entry

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery PubMed

Database entries

• Structure: 1YGM PubMed

• UniProt: Q5BU39

• KEGG entry: [1]

• E.C. number:

Expression and regulation

• Operon: mstX-yugO PubMed

• Sigma factor:

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Matthew E Lundberg, Eric C Becker, Senyon Choe
MstX and a putative potassium channel facilitate biofilm formation in Bacillus subtilis.
PLoS One: 2013, 8(5);e60993
[PubMed:23737939] [WorldCat.org] [DOI] (I e)

Dilip K Debnath, Rajiv Vaid Basaiawmoit, Kåre Lehmann Nielsen, Daniel E Otzen
The role of membrane properties in Mistic folding and dimerisation.
Protein Eng Des Sel: 2011, 24(1-2);89-97
[PubMed:21097953] [WorldCat.org] [DOI] (I p)

Hay Dvir, Matthew E Lundberg, Samir K Maji, Roland Riek, Senyon Choe
Mistic: cellular localization, solution behavior, polymerization, and fibril formation.
Protein Sci: 2009, 18(7);1564-70
[PubMed:19475664] [WorldCat.org] [DOI] (I p)

Tarmo P Roosild, Mark Vega, Samantha Castronovo, Senyon Choe
Characterization of the family of Mistic homologues.
BMC Struct Biol: 2006, 6;10
[PubMed:16704729] [WorldCat.org] [DOI] (I e)

Tarmo P Roosild, Jason Greenwald, Mark Vega, Samantha Castronovo, Roland Riek, Senyon Choe
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.
Science: 2005, 307(5713);1317-21
[PubMed:15731457] [WorldCat.org] [DOI] (I p)