PAC7

pAC7: click to enlarge

pAC7 cloning region: click to enlarge

vector for the construction of translational lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: kanamycin resistance

similar vectors: pAC5, pAC6

sequencing primer:

• PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
• PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
• Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘