BglP

• Description: trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBCA of the PTS
  
  

Gene name	bglP
Synonyms	sytA
Essential	no
Product	trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBCA
Function	beta-glucoside uptake and phosphorylation, control of LicT activity
Gene expression levels in SubtiExpress: bglP
Interactions involving this protein in SubtInteract: BglP
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	64 kDa, 6.809
Gene length, protein length	1827 bp, 609 aa
Immediate neighbours	bglH, yxxE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, LicT regulon

The gene

Basic information

• Locus tag: BSU39270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, sucrose permease (Scr) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • BglP-HPr, BglP-LicT

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: P40739

• KEGG entry: [3]

• E.C. number: 2.7.1.69 9

Expression and regulation

• Operon: bglP-bglH-yxiE PubMed

• Expression browser: bglP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA, LicT) PubMed
  • induced by salicin (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression, (CcpA binding site overlaps -35 region) PubMed
  • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr PubMed

• Additional information:
  • An antisense RNA is predicted for bglP PubMed

Biological materials

• Mutant: GP475 (erm), available in the Stülke lab

• Expression vector:
  • pGP1290 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab,
  • pGP1300 (expression of bglP in B. subtilis, in pBQ200), available in Stülke lab

• lacZ fusion: pGP600 (in pAC6), available in Stülke lab

• GFP fusion:

• CFP fusion: B. subtilis GP1266 bglP-cfp ermC- without terminator, available in Jörg Stülke's lab

• YFP fusion: B. subtilis GP1274 bglP-yfp ermC- without terminator, available in Jörg Stülke's lab

• two-hybrid system:

• Antibody: