GuaC

• Description: GMP reductase
  
  

Gene name	guaC
Synonyms	yumD
Essential	no
Product	GMP reductase
Function	purine salvage and interconversion
Gene expression levels in SubtiExpress: guaC
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	35 kDa, 6.078
Gene length, protein length	978 bp, 326 aa
Immediate neighbours	yuzG, paiB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

CodY regulon, PurR regulon

The gene

Basic information

• Locus tag: BSU32130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Inosine 5'-phosphate + NH₃ + NADP⁺ = guanosine 5'-phosphate + NADPH (according to Swiss-Prot)

• Protein family: GuaC type 2 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YPF (from Bacillus anthracis, 84% identity, 91% similarity)

• UniProt: O05269

• KEGG entry: [3]

• E.C. number: 1.7.1.7

Expression and regulation

• Operon: guaC PubMed

• Expression browser: guaC PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • expression activated by glucose (3.3 fold) PubMed

• Regulatory mechanism:
  • CodY: transcription activation PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3 to 20 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon.
J Biol Chem: 1989, 264(6);3553-61
[PubMed:2536750] [WorldCat.org] (P p)