GlnH

• Description: glutamine ABC transporter (binding protein)
  
  

Gene name	glnH
Synonyms
Essential	no
Product	glutamine ABC transporter (binding protein)
Function	glutamine uptake
Gene expression levels in SubtiExpress: glnH
Interactions involving this protein in SubtInteract: GlnH
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	29 kDa, 8.549
Gene length, protein length	819 bp, 273 aa
Immediate neighbours	glnQ, glnM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, glutamate metabolism, utilization of amino acids, membrane proteins, sporulation proteins

This gene is a member of the following regulons

SigE regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU27440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K(D) for Gln: 120 myM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GlnQ-(GlnM-GlnP)-GlnH PubMed

• Localization: attached to the membrane via GlnM-GlnP PubMed

Database entries

• Structure:

• UniProt: O34563

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glnQ-glnH-glnM-glnP PubMed

• Expression browser: glnH PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • expressed during sporulation (SigE) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP734 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)