AlaS

• Description: alanine-tRNA synthetase
  
  

Gene name	alaS
Synonyms
Essential	yes PubMed
Product	alanine-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: alaS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	97 kDa, 4.922
Gene length, protein length	2634 bp, 878 aa
Immediate neighbours	yrzL, yrrI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU27410

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YFS (enzyme from Aquifex aeolicus) PubMed, 3HXU (enzyme from E. coli with alanine) PubMed

• UniProt: O34526

• KEGG entry: [2]

• E.C. number: 6.1.1.7

Expression and regulation

• Operon:

• Expression browser: alaS PubMed

• Sigma factor:

• Regulation:
  • induced by alanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: