CwlA

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlA
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	minor autolysin
Gene expression levels in SubtiExpress: cwlA
MW, pI	29 kDa, 10.4
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	yqxI, yqxH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, Skin element

The gene

Basic information

• Locus tag: BSU25900

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_2 domain (like BlyA, CwlH, XlyA, XlyB)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P24808

• KEGG entry: [2]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:

• Expression browser: cwlA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

A Kuroda, M Imazeki, J Sekiguchi
Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.
FEMS Microbiol Lett: 1991, 65(1);9-13
[PubMed:1908396] [WorldCat.org] [DOI] (P p)